Tris(hydroxymethyl)aminomethane

☑ Ehara i te mea ka whakamahia whanuitia te Tris buffer hei whakarewa mo nga waikawa karihi me nga pūmua, engari he maha ano nga whakamahinga nui.I whakamahia a Tris mo te tipu karaihe pūmua i raro i nga ahuatanga pH rereke.
☑ Ka taea te whakamahi i te kaha katote iti o Tris buffer ki te hanga i te muka takawaenga o te lamin i roto i te C. elegans.
☑ Ko Tris hoki tetahi o nga waahanga matua o te pūmua electrophoresis buffer.
☑I tua atu, he takawaenga a Tris mo te whakareri i nga hau, nga kaiwhakatere whakakino me etahi raau taero.Ka whakamahia ano a Tris hei paerewa titration.
☑Ka whakamahia nuitia te Trimethylaminomethane i roto i te mate huka me te waikawa manawa.He parepare kawakore, he pai hoki te awe ki runga i te waikawa metabolic me te tauhohenga ngohe ngohe.
☑He maha nga wa e whakamahia ana a Tris hei parenga koiora, ko tona uara pH he 6.8, 7.4, 8.0, 8.8.Ko tana hanganga, he rereke te uara pH ki te pāmahana.Ko te tikanga, ka heke te uara pH ma te 0.03 ka piki te pāmahana ma te tohu kotahi.Kei te whakamahia nuitia a Tris ki te whakarite i te otinga parepare i roto i te matū koiora me nga whakamatautau koiora rapoi.Hei tauira, ko te TAE me te tbe buffers (whakamahia mo te whakakore waikawa karihi) e whakamahia nuitia ana i roto i nga whakamatautau matū koiora me Tris.Na te mea kei roto he roopu amino, ka taea te tauhohe me te aldehydes.
☑ He turanga ngoikore a Tris, ko tana PKA he 8.1 i te pāmahana rūma (25 ℃).E ai ki te ariā parepare, kei waenganui i te 7.0 me te 9.2 te awhe parepare whai hua o Tris buffer.Ko te uara pH o te wairewa wai turanga Tris he 10.5 pea.Ko te tikanga, ka taapirihia te waikawa maaota ki te whakatika i te uara pH ki te uara e hiahiatia ana kia whiwhi ai i te otinga parepare me tenei uara pH.I te wa ano, me aro tatou ki te paanga o te pāmahana ki te pKa o Tris.Na te mea he otinga kawakore ngoikore a Tris buffer, ka whakahekehia te DNA i roto i taua otinga hei whakapai ake i tona whakarewatanga.He maha nga wa ka taapirihia e te tangata te EDTA ki roto i te parepare waikawa maataota o Tris hei hanga "Te buffer", e whakamahia ana mo te whakapumau DNA me te rokiroki.Mena ka whakakapihia te wairewa waikawa o te whakatika i te uara pH ki te waikawa acetic, ka whiwhi te "Tae buffer" (Tris / acetate / EDTA), ka whiwhi te "tbe buffer" (Tris / borate / EDTA) ma te whakakapi ki te waikawa boric .Ka whakamahia enei parepare e rua ki nga whakamatautau electrophoresis waikawa karihi.